What is RPMI culture medium?
RPMI 1640, also known as RPMI medium, is a growth medium used in cell culture. RPMI 1640 was developed by George E. Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human T/B-lymphocytes, bone marrow cells, and hybridoma cells.
What is RPMI medium used for?
RPMI is used when laboratory testing requires fresh, unfixed specimens such as flow cytometry as well as some FISH, molecular and cytogenetics tests.
What kind of cell types is RPMI medium used for?
RPMI is suitable for cells in suspension or monolayer and is widely used to grow a variety of mammalian cell types. These include primary human leukocytes, bone marrow cells, hybridomas, as well as common cell lines.
What does RPMI test for?
RPMI agar is a culture media specially formulated for carrying out antifungal susceptibility testing of yeasts and molds when using Etest® method. Recommended for the antifungal susceptibility testing with Etest® caspofungin, fluconazole, flucytosine, ketoconazole, voriconazole, itraconazole, amphotericin B.
Can I use Dmem instead of RPMI?
On a very basic level in our laboratory you would use DMEM for an adherent cell line and you would use RPMI for suspension cell lines. For example some cell lines grow and flourish better in the presence of HEPEs. Also you need to think about your down the line uses for the cells.
What does RPMI 1640 stand for?
Roswell Park Memorial Institute
RPMI 1640 is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966. This formulation of RPMI 1640 comes without L-Glutamine. RPMI 1640 was developed to support lymphoblastoid cells in suspension cultures.
What is the difference between RPMI and DMEM?
RPMI is a media used widely to culture mammalian cells in suspension culture. DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.
How long can a Specimen stay in RPMI?
Specimens are stable up to 24 hours at 2-8ºC in RPMI. Specimens which have had fixative added (i.e., formalin/Pap fixative) cannot be used for flow cytometry. Bone marrow: Collect 1-3 ml aspirated marrow in EDTA (sodium heparin or ACD are also acceptable).
Can I use DMEM instead of RPMI?