What is NuSieve GTG agarose?
NuSieve® GTG® Agarose is a unique low melting temperature agarose for resolving DNA fragments from 10 bp to 1,000 bp. NuSieve® GTG® Agarose is tested and certified for reliable ligation and transformation of DNA directly in remelted agarose.
What is low melting point agarose?
Low Melting (LM) Agarose is a low melting temperature agarose with the highest resolving capacity for large DNA fragments, including PCR products. Low melting temperature allows for the recovery of undamaged nucleic acids below denaturation temperature.
What is the difference between standard agarose and low melting agarose?
LM agaroses have higher clarity (gel transparency) than gels of standard agaroses. LM agaroses have great sieving capacity. The gelling temperature of LM agaroses is 24 to 28°C. The gels have a macroreticular structure with a very open mesh which can be adjusted simply by varying the concentration of the agarose.
What is low EEO agarose?
Agarose I (All Purpose, Electrophoresis Matrix (Low EEO)) G-Biosciences. Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility. Suitable for nucleic acid analytical and preparative electrophoresis; blotting; and protein electrophoresis (i.e. Radial Immunodiffusion).
What is lowest melting point?
The chemical element with the lowest melting point is Helium and the element with the highest melting point is Carbon. The unity used for the melting point is Celsius (C).
How do you make low melting agarose?
To melt the agarose in solutions of less than 2%, heat the slurry in microwave oven on high power setting until it starts to boil. Allow the solution to boil for 1 min or until the solution is clear and all particles are dissolved. Remove the flask from the microwave oven, and gently swirl to mix the agarose solution.
How do you do a comet assay?
Comet assay utilizes single cells to measure DNA damage. First, cells are embedded into agarose and then placed onto a slide. The slide is then immersed into lysis solution to break open the cell membrane. After the cells are lysed, DNA is denatured under neutral or alkaline conditions and run through electrophoresis.
How do you melt an agarose?
What is melted agarose poured into?
To make a gel, agarose powder is mixed with an electrophoresis buffer and heated to a high temperature until all of the agarose powder has melted. The molten gel is then poured into a gel casting tray and a “comb” is placed at one end to make wells for the sample to be pipetted into. Many people now use pre-made gels.
What is the lowest melting metal?
What metal has the lowest melting point? At the lower end is lead, which melts at the relatively low temperature of 621 °F / 327 °C.
What is the difference between high melting point and low melting point?
The melting point of a pure substance is always higher and has a smaller range than the melting point of an impure substance or, more generally, of mixtures. The higher the quantity of other components, the lower the melting point and the broader will be the melting point range, often referred to as the “pasty range”.
How do you use low melting point agarose?
Low melting point agarose gels should be poured and allowed to set up in the cold room, but electrophoresed at room temperature. The gels should be stained at 4°C and kept cold until ready to cut out the DNA bands from the gel. The gels will be fragile, so handle gently. Heat the tubes at 65°C for 10-15 min.