What is immunophenotyping for leukemia?
With immunophenotyping, a blood, bone marrow, or other sample can be tested to gather this information – information that is then used to identify a specific type of leukaemia or lymphoma and, where possible, used to predict its likely aggressiveness and/or responsiveness to certain treatment.
What is the importance of immunophenotyping?
Immunophenotyping can be a useful tool to detect small numbers of residual leukaemic cells in peripheral blood or bone marrow specimens when such cells are not detected by standard morphology or histopathology.
What are the cell surface markers used to differentiate AML from ALL?
A major diagnostic utility of mo abs lies in distinguishing AML from ALL. Monoclonal antibodies CD 13 and CD 33 are used as myeloid markers [12, 21]. Neame et al reported CD 13 and CD 33 to be positive in 96% of the cases of AML [16].
What is immunophenotyping in flow cytometry?
Immunophenotyping by flow cytometry is a laboratory method that detects the presence or absence of white blood cell (WBC) markers called antigens. These antigens are protein structures found on or within WBCs. Additionally, specific patterns of antigens are present on abnormal cells seen in leukemias and lymphomas.
How is a immunophenotyping test done?
Immunophenotyping is a test in which the cells in a sample of blood or bone marrow are looked at under a microscope to find out if malignant lymphocytes (cancer) began from the B lymphocytes or the T lymphocytes. The process identifies cells based on the types of antigens or markers on the surface of the cell.
What is Immunophenotypic analysis?
Immunophenotyping is the analysis of heterogeneous populations of cells for the purpose of identifying the presence and proportions of the various populations of interest.
What is Immunophenotypic evidence?
Immunophenotyping is a test used to identify cells on the basis of the types of markers or antigens present on the cell’s surface, nucleus, or cytoplasm. This technique helps identify the lineage of cells using antibodies that detect markers or antigens on the cells, hence the “immuno-” prefix.
What is CD10 a marker for?
CD10 is a sensitive and diagnostically useful immunohistochemical marker of normal endometrial stroma and of endometrial stromal neoplasms.
What does pre B all mean?
Pre-B-cell ALL In between 75-80% of adult cases, ALL arises in B-lymphocytes in the early stages of development in the bone marrow. The disease is therefore called precursor B-cell ALL or Pre-B-cell ALL.
When do you use immunophenotyping?
When should it be ordered? In a primary care setting, flow immunophenotyping is generally ordered: To investigate persistent, unexplained lymphocytosis. To characterize circulating abnormal lymphocytes or other mononuclear cells (e.g.: blast cells) which could indicate a peripheralized lymphoma or an acute leukemia.
What is peripheral immunophenotyping?