What is IFN gamma Elisa?
The Human Interferon-gamma (IFN-γ) ELISA Kit is designed for the quantitative detection and measurement of human IFN-γ in biological fluids such as serum, plasma, and cell culture supernatants. IFN-γ is primarily secreted by T and NK cells, but is also produced by dendritic cells and macrophages in smaller amounts.
What is IFN gamma used for?
Interferon gamma-1b injection is used to reduce the frequency and severity of serious infections in people with chronic granulomatous disease (an inherited immune system disease). It is also used to slow down worsening of their condition in people with severe, malignant osteopetrosis (an inherited bone disease).
What is IFN gamma act?
IFN-γ implements a broad spectrum of effects on immune responses, including activation of monocytes, and when produced in excess can promote tissue injury. Among its activities are the induction of other proinflammatory cytokines such as TNF and induction of apoptosis in renal parenchymal cells.
What type of IFN is IFN gamma?
Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons.
Is IGRA the same as QuantiFERON?
QuantiFERON-TB Gold (QFT) is a simple blood test that aids in the detection of Mycobacterium tuberculosis, the bacteria which causes tuberculosis (TB). QFT is an interferon-gamma (IFN-γ) release assay, commonly known as an IGRA, and is a modern alternative to the tuberculin skin test (TST, PPD or Mantoux).
How does IFN-gamma work?
Specifically, IFN-γ plays a major role in activating anticancer immunity, by promoting the activity of CD4 T helper type 1 cells, CD8 cytotoxic T lymphocyte (CTL), natural killer (NK) cells, dendritic cells (DCs), and macrophages, promoting the antigen presentation.
What cells express IFN gamma?
Interferon-gamma is secreted predominantly by activated lymphocytes such as CD4 T helper type 1 (Th1) cells and CD8 cytotoxic T cells (23–26), γδ T cells (27–33), and natural killer (NK) cells (34, 35) and, to a less extent, by natural killer T cells (NKT), B cells (36–39), and professional antigen-presenting cells ( …
Is IFN gamma A Type 1 IFN?
IFN-γ, like type I IFN, promotes antiviral immunity through its regulatory effects on the innate immune response and acts as a key link between the innate immune response and activation of the adaptive immune response (3).
What is TB Gold?
What if TB Gold test is negative?
Negative – suggests that TB infection is unlikely. Indeterminate – suggests the need for further investigations or repeat testing.
Which cells express IFN-gamma?
Which is the ELISA assay for Hu IFNγ?
Created with sketchtool. The Human Interferon gamma (Hu IFNγ) ELISA quantitates Hu IFNγ in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Hu IFNγ.
What are the results of the IFN gamma kit?
Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.
How does the quantikine human IFN-gamma immunoassay work?
The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma.
How is the linearity of the IFN gamma assay evaluated?
The recovery of IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated. To assess the linearity of the assay, five samples were spiked with high concentrations of IFN-gamma in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.