What is eg7 cells?
ATCC eg7 cell line. Applications This cell line is a model system for studying major histocompatibility complex MHC class I restricted responses of cytotoxic T lymphocytes in mice Cell Type T lymphocyte Host Mus musculus mouse.
What is B16 ova?
B16-OVA MO4 cell line is a model for cancer immunotherapy, expressing ovalbumin (OVA) in order to facilitate strong immune responses to tumor antigens. More>> B16-OVA MO4 cell line is a model for cancer immunotherapy, expressing ovalbumin (OVA) in order to facilitate strong immune responses to tumor antigens.
What are B16 cells?
B16 melanoma is a murine tumor cell line used for research as a model for human skin cancers. B16 cells are useful models for the study of metastasis and solid tumor formation, and were one of the first effective murine tools for metastasis research.
Where are B16 cells derived from?
B16 cells originate in melanin-producing epithelia of mice, and are easy to track in vivo post-transplantation. Their fidelity of metastasis from skin to lung, liver, and spleen make them useful and predictable tools to study metastatic pathways.
What is B16F10 cell line?
Description. B16F10 (ATCC® CCL-6475™) is a murine melanoma cell line from a C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, and injecting them into fresh mice, a total of 10 times.
What is suitable media for B16F10 cell line?
B16F10 Cell Line
General Information | |
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Organism | Mus musculus, mouse |
Culture Medium | 90% Dulbecco’s modified Eagle’s medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum. |
What is MC38?
MC38 (Corbett el al., Can Res, 1975) is a murine colon adenocarcinoma cell line from a C57BL/6 mouse. The cells are adherent and have a fibroblast morphology. MC38 cells will form tumors and metastases post implantation into syngeneic C57BL/6 mice or immunocompromised mice.
Is ovalbumin globular or fibrous?
Ovalbumin is an example of a globular protein, one that is water-soluble and shaped roughly like a globe or a sphere when coiled up into its functional form.
Why is ovalbumin used in vaccines?
Ovalbumin (OVA) is a key reference protein for vaccination experiments. Ovalbumin, the major protein constituent of chicken egg whites, is a glycoprotein that is sufficiently large and complex to be mildly immunogenic. Consequently, it is widely used as an antigen for immunization research.
Which is the eg7 cell line supplied by ATCC?
Eg7 Ova Cell Line El4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS – scores, article reviews, protocol conditions and more
Is the eg7 cell line a stable transfectant?
The EG7 cell line (American Type Culture Collection, CRL-2113), which is a stable transfectant of the murine OVA-expressing EL4 thymoma (H-2b), was maintained in complete RPMI 1640 medium supplemented with G418 (0.4 mg/mL; Calbiochem® , Merck Millipore, Darmstadt, Germany) for the tumor challenge study.
Which is OVA expressing thymoma cell line eg7?
Ova Expressing Thymoma Cell Line Eg7, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS – scores, article reviews, protocol conditions and more Article Title: OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice
Where can I get eg7 cell culture from?
EG7 cells, purchased from ATCC (American Type Culture Collection, Manassas, VA, USA), were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 55 µM 2-mercapto-ethanol (2-ME), 1 mM sodium pyruvate, 100 U/mL penicillin, and 100 µg/mL streptomycin (Life Technologies, Carlsbad, CA, USA).