What does Flox mean?
Blend of fluorine and liquid oxygen.
How do you Flox a gene?
In essence, through the use of floxing and Cre recombinase, it is possible to splice a gene entirely out of the genome, invert the orientation of the gene by removing it and splicing it back in with the opposite orientation, and replace the gene with a floxed DNA sequence that originated elsewhere.
Is Flox a word?
No, flox is not in the scrabble dictionary.
What are Flox Flox mice?
HTT flox/flox (or HTT Fl/Fl) refers to the idea that the mice mentioned above have a knockin of the HTT gene in which portions of the gene are flanked by two Cre recombinase recognition sites. Cre recombinase sites would be positioned upstream (possibly the distal promoter region) and downstream (possibly Intron 1).
How do you spell Flox?
noun A simplified spelling of phlox .
How does Flox CRE work?
How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.
How are loxP sites introduced?
The loxP sites can be introduced into the genomic locus of interest by homologous recombination as described above. When crossing the floxed mouse with a Cre transgenic mouse, the floxed DNA sequence is subsequently deleted in the cell types or tissues, where Cre is expressed.
Is CLOX a scrabble word?
clox is an acceptable dictionary word for games like scrabble, words with friends, crossword, etc. Using the word ‘clox’ in Scrabble will fetch you -1 points while using it in Words with Friends will fetch you -1 points (without taking into consideration the effect of any multipliers).
How are mice floxed?
The Cre-lox system allows for the deletion of a gene in mouse ES cells and its inversion or complete inactivation. This process is called “floxing” since it combines flanking the gene with the help of its adjacent two loxP sites and the Cre-lox recombination system.
How are floxed mice generated?
Traditionally, flox mice have been obtained by gene targeting in embryonic stem cells followed by production of germline chimeric mice.
Which is the best definition of the word Flox?
( third-person singular simple present floxes, present participle floxing, simple past and past participle floxed) (molecular biology) To sandwich a DNA sequence between two recombinase binding sequences such as “loxP”
Where does the term floxing come from in genetics?
In genetics, floxing refers to the sandwiching of a DNA sequence (which is then said to be floxed) between two lox P sites. The terms are constructed upon the phrase “flanking/flanked by LoxP”.
What’s the alternative capitalization for the word Flox?
(molecular biology) To sandwich a DNA sequence between two recombinase binding sequences such as “loxP” Alternative capitalization of FLOX. (astronautics, dated) To add fluorine to liquid- oxygen rocket fuel.
How can floxing be used to study cancer?
For example, using the Cre-lox system, researchers can study oncogenes and tumor suppressor genes and their role in development and progression of cancer in mice models. Floxing a gene allows it to be deleted (knocked out), translocated or inserted (through various mechanisms in Cre-Lox recombination).
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