Why are THP-1 cells used?
THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry.
Are THP-1 macrophages?
THP-1 cells can be differentiated into macrophage-like cells that resemble properties of mature macrophages by activation of protein kinase C (PKC) with phorbol-12-myristate-13-acetate (PMA), ultimately resulting in cells with increased adherence and loss of proliferative activity (Schwende et al., 1996).
What does PMA do to macrophages?
PMA at moderate concentrations induced apoptosis in macrophages, and this process appeared to be increased in the presence of myelin. In contrast, microglia were activated by PMA, and greatly increased their phagocytosis of myelin.
Do THP-1 cells express CD14?
As opposed to primary monocytes, THP-1 cells express low levels of CD14 (9). Therefore, concerning LPS responses, THP-1 cells appear to be a poor model for primary monocytes.
How do you maintain THP-1 cells?
THP-1 cells need cell-to-cell contact to grow, so keep them in T25 or T75 flasks around 5×10^5/1×10^6 cells/ml. Moreover, do not change medium when you split cells! Just add fresh new medium to the old one, otherwise you’ll lose essential substances produced by the cells themselves!
Are THP-1 cells adherent?
Namely, increased phagocytic activity, HLA-DR expression and MLR stimulator activity were remarkable. This adherent cell line was designated as activated-THP-1 (A-THP-1), since it demonstrated characteristics of activated macrophages continuously without exogenous stimulation.
Are THP 1 cells adherent?
How do you freeze down a THP-1 cell?
To Successfully Freeze THP-1 Cells, Follow These Tips:
- Thaw frozen vials in a 37°C water bath, but DO NOT LET THE WATER COME IN CONTACT WITH THE LID.
- Transfer the cells to a flask containing warmed media, then transfer some of this back into the vial to get as many cells as you can.
What is Vero cell culture?
Vero cells are a lineage of cells used in cell cultures. The ‘Vero’ lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species).
What does PMA do to monocytes?
When exposed to the phorbol ester PMA, THP-1 monocytes differentiate to mature macrophages. Our results have demonstrated that although THP-1 cells respond to LPS by the secretion of TNF-α, the induction of differentiation enhanced TNF-α transcription and secretion.
Are Jurkat CD4 or CD8?
Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR. The T2 line is a TAP-deficient T-cell/B-cell hybridoma expressing HLA-A2.
What is the phagocytic activity of THP-1 cells?
Here we investigated phagocytic plasticity of THP-1 (acute monocytic human leukemia) cells at different stages (days 1, 3, and 7) of phorbol ester (PMA)-induced macrophage differentiation. Analysis of cytokine profiles showed that PMA at a concentration of 100 nM induced development of the proinflammatory macrophage population.
How are THP-1 monocytes similar to macrophages?
THP-1 monocytes stimulated by phorbol-12-myristate-13-acetate (PMA) are regarded as macrophage-like cells, showing similarities with primary macrophages in morphology, gene transcription and expression ( 13 – 16 ). There are only few reports describing the differentiation of THP-1 monocytes into dendritic-like cells.
How are THP-1 derived macrophages uptake β lactoglobulin?
The uptake of β-lactoglobulin after wet-heating (60°C in solution) by THP-1 derived macrophages was earlier reported to be significantly increased.
What is the role of macrophages in phagocytosis?
Studies of the role of macrophages in phagocytosis are of great theoretical and practical importance for understanding how these cells are involved in the organism’s defense response and in the development of various pathologies. Here we investigated phagocytic plasticity of THP-1 (acute monocytic h …