What is the resin in the Hitrap Q column?
Composed of crosslinked 6% agarose beads with quaternary amine strong anion exchange groups, this resin has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. The resin’s 90 µm bead size yields rapid flow rates, making it simple to scale up the purification.
How do you clean an ion exchange column?
- Wash with 2 column volumes of detergent in a basic or acidic solution, e.g. 0.1–0.5% non-ionic detergent in 0.1 M acetic acid (see Table 21 for recommended flow).
- Rinse with 5 column volumes 70% ethanol to remove residual detergent (see Table 21 for recommended flow).
What is Mono Q column?
Mono Q® 5/50 GL. Applications Products Services Support. All Photos(1) GE17-5166-01.
What soil has the highest cation exchange capacity?
Clay. Clay has a great capacity to attract and hold cations because of its chemical structure. However, CEC varies according to the type of clay. It is highest in montmorillonite clay, found in chocolate soils and black puggy alluvials.
How to wash HiTrap Q XL with water?
HiTrap Q XL: Rinse with water then wash with 5 column volumes 20% ethanol at 1 ml/min (HiTrap 1 ml column) or at 5 ml/min (HiTrap 5 ml column) to prevent microbial growth. HiTrap SP XL: Rinse with water then wash with 5 column volumes 20% ethanol containing 0.2 M sodium acetate at 1 ml/min (HiTrap
What are the chains in HiTrap Q XL?
Q Sepharose XL and SP Sepharose XL resins have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.
When to use HiTrap SP XL and 5 ml?
HiTrap SP XL and HiTrap Q XL are 1 ml and 5 ml prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules. HiTrap SP XL and HiTrap Q XL provide fast, reproducible, and easy separations in a convenient format.