What is propidium iodide used for?
Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI binds to DNA by intercalating between the bases with little or no sequence preference.
Can propidium iodide stain live cells?
Propidium iodide is a vital stain. a live cells exclude the dye and cannot be stained. It will also be dependant on the concentration of PI that you are using.
Is propidium iodide toxic?
CONSIDERED A HAZARDOUS SUBSTANCE ACCORDING TO OSHA 29 CFR 1910.1200. Harmful if swallowed. Possible risk of irreversible effects. Irritating to eyes, respiratory system and skin.
Is propidium iodide cytotoxic?
A propidium iodide fluorescence assay (PIA) was developed to characterize the in vitro growth of human tumor cell lines as well as to test the cytotoxic activity of standard compounds. Propidium iodide (PI) was used as a dye which penetrates only damaged cellular membranes.
What color is propidium iodide?
red-fluorescent
Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.
Why is propidium iodide used in flow cytometry?
Flow Cytometry Cell Viability Overview Flow cytometry is a quick and reliable method to quantify viable cells. Propidium iodide (PI) is a membrane impermeant dye that is generally excluded from viable cells. It binds to double stranded DNA by intercalating between base pairs.
How is propidium iodide used as a dye?
Propidium iodide is an organic iodide salt. It contains a propidium. Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
What is the molecular weight of propidium iodide?
Propidium iodide PubChem CID 104981 Synonyms propidium iodide 25535-16-4 Propidium di Molecular Weight 668.4 Parent Compound CID 4939 (Propidium) Component Compounds CID 4939 (Propidium) CID 24841 (Hydriodi
Are there any synonyms for propidium iodine?
Synonyms. propidium iodide. 25535-16-4. Propidium diiodide. 3,8-Diamino-5- (3- (diethyl (methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide. Propidium Iodine. More… Molecular Weight. 668.4 g/mol.
How does propidium iodide ( PI ) bind to DNA?
Propidium iodide (PI) binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4–5 base pairs of DNA.1PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining.