What is H358 cell line?
H358 is a non-small cell lung cancer (NSCLC) cell line that harbors a KRAS mutation. KRAS mutations are found in >25% of lung adenocarcinomas leading to constitutive activation of the growth-promoting RAS signaling pathway. H358 cells were established from a chemotherapy-naïve NSCLC tumor.
What are the subtypes of NSCLC?
There are three main subtypes of non-small cell lung cancer (NSCLC), including the following: Squamous cell carcinoma (25% of lung cancers). Adenocarcinoma (40% of lung cancers). Large cell carcinoma (10% of lung cancers).
Is A549 a Nsclc?
The cells are able to synthesize lecithin and contain high levels of unsaturated fatty acids, which are important to maintain membrane phospholipids. A549 cells are widely used as a type II pulmonary epithelial cell model for drug metabolism and as a transfection host.
What are calu 3 cells?
Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene. They express wildtype EGFR and mutant K-Ras (G13D). In addition, they harbor mutations in TP53 and CDKN2A genes.
What is first line treatment for NSCLC?
In advanced NSCLC, chemotherapy is recommended as first-line treatment in patients with good performance status. Treatment objectives are survival, quality of life and symptom control improvement. Cisplatin-based chemotherapy with one of the effective regimens should be used.
What are Huh 7 cells?
Huh-7 is an immortal cell line composed of epithelial-like, tumorigenic cells. The majority of Huh-7 cells show a chromosome number between 55 and 63 (mode 60) and are highly heterogeneous. These cells are adherent to the surface of flasks or plates and typically grow as 2D monolayers.
What are BEAS 2B cells?
BEAS-2B cells were derived from normal bronchial epithelium obtained from autopsy of non-cancerous individuals. The cell line has been applied for studies of pneumococcal infection mechanisms. BEAS-2B was described to express keratins and SV40 T antigen.
Can a h358 NSCLC cell form a tumor?
Conversely, Hera Biolabs has demonstrated that H358 cells form rapid, successful xenografts in humanized rats that are Rag2 knockout and deficient in B- and T- cells (SDR rats). These once culture-defiant, NSCLC cells form tumors with volumes between 2000-3000 mm 3 within 25 days.
What kind of lung cancer is h358?
H358 is a non-small cell lung cancer (NSCLC) cell line that harbors a KRAS mutation. KRAS mutations are found in >25% of lung adenocarcinomas leading to constitutive activation of the growth-promoting RAS signaling pathway. H358 cells were established from a chemotherapy-naïve NSCLC tumor.
Is the h358 cell line an oncogenic mutation?
Interestingly, H358 cells are highly sensitive to anti-EGFR therapies, despite having an oncogenic KRAS activation mutation. Additionally, H358 has no other major tumor suppressor or oncogenic mutations. This makes H358 an imperative NSCLC cell line model for trying to understand the complex role of KRAS mutations in NSCLC.
Which is NSCLC cell line harbors KRAS mutation?
Oncogenic KRAS is a known anti-EGFR-drug-resistance mechanism. The NSCLC cell line, H358 (AKA: NCI-H385, H-358), harbors a heterozygous missense KRAS mutation at codon 12 (G12C) which leads to constitutive activation of the EGFR-KRAS signaling cascade in H358 cells. Over 40% of KRAS mutant NSCLC have the G12C KRAS mutation found in H358 cells.