What is DharmaFECT?
DharmaFECT is the only transfection reagent offered in four distinct siRNA-specific formulations to ensure optimal siRNA delivery in a wide range of cell lines. No single lipid reagent outperformed the panel of DharmaFECT formulations in a. benchmark study of twenty common cell lines.
How much siRNA do I need?
Pre-designed siRNA’s – How Much Do I Need?
| Number of Transfections 20 nmol of siRNA | ||
|---|---|---|
| 6 well (2.5 ml) | 800 | 80 |
| 12 well (1 ml) | 2000 | 200 |
| 24 well (0.5 ml) | 4000 | 400 |
| 96 well (0.1 ml) | 20,000 | 2000 |
Can I Vortex siRNA?
Incubate the diluted siRNA mixture at room temperature for 5 min. Important: do NOT vortex the diluted siRNA mixture. Incubate at room temperature for 5-30 minutes (5 minutes is typically sufficient).
How do siRNA work?
The siRNA molecule halts the production of amyloid proteins by interfering with the RNA production of abnormal TTR proteins. This prevents the accumulation of these proteins in different organs of the body and helps the patients manage this disease.
How many cell types are dharmafect 1 validated in?
DharmaFECT 1 has been validated in over 35 cell types. Updated shipping conditions: Horizon Discovery always strives to reduce its environmental impact. Sustainability goals factor in many decisions regarding our business.
What are the different types of dharmafect transfection?
DharmaFECT 2, 3, and 4 offer distinct formulations to support a wider range of cell types, and permit more thorough optimization of transfection for high-value experiments and screening projects. Use the DharmaFECT Cell Type Guide to review validated formulations for common cell types.
Which is Dharma fect 1 siRNA transfection reagent?
Dharma FECT ® 1 siRNA transfection reagent From Dharmacon DharmaFECT® 1 siRNA transfection reagent is specifically formulated for the following cell lines: A549, HEK293, HeLa, HeLa 53, MCF7, DU 145, HUVEC, SKBR3 and H9C2 cells.
Which is better dharmafect siRNA or Lipofectamine 2000?
DharmaFECT siRNA transfection reagents ensure effective delivery across a broad range of cell densities and lipid volumes with minimal effect on cell viability. DharmaFECT reagents are effective across a broader range of experimental conditions when compared to Lipofectamine 2000 (Invitrogen).