What does f480 stain for?
The antibody binds to macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. …
How are macrophages stained?
Staining pattern: The macrophages are stained red and are easy to identify in the light background. An example of the CD68 immunostaining is shown in Fig 1. Atherosclerotic lesions were immunostained for macrophage specific marker CD68 reddish-(brown stain) in ApoE−/− mice fed western diet for 16 weeks.
Does F4 80 stain microglia?
Characteristics of the anti-F4/80 antibody clone CI:A3-1 In addition to recognizing kupffer cells, langerhans cells, peritoneal macrophages, and splenic red pulp macrophages, the anti-F4/80 antibody clone CI:A3-1 is distinguished from other F4/80 monoclonal antibodies in that it stains microglia (macrophages of the …
What cells express f480?
F4/80 is highly and constitutively expressed on most resident tissue macrophages, including the red pulp macrophages in the spleen, microglia in the brain, Kupffer’s cells in the liver, and Langerhans’ cells in the skin (6).
Do macrophages express CD11c?
It is now recognized that several non-DC myeloid populations express CD11c, including alveolar macrophages and resident (Ly6C low) monocytes and that some macrophage populations express both CD11c and MHC class II [8, 9]. Similarly, CD11b has often been used as a marker for all myeloid cell types.
Is F4 80 expressed in human macrophages?
F4/80 is expressed at high levels on the surface of various macrophages: kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and langerhans cells in the skin.
Do eosinophils express f480?
Monocytes are F4/80low and most of the monocyte-derived macrophages are F4/80low. Eosinophils express F4/80 as well (though less than macrophages).
How is F4 / 80 used as a macrophage marker?
The F4/80 molecule was established as a unique marker of murine macrophages when a monoclonal antibody was found to recognize an antigen exclusively expressed by these cells. However, recent research has shown that F4/80 is expressed by other immune cells and is not equivalently expressed across tissue-specific macrophage lineages,
Which is the best F4 / 80 antibody for microglia?
F4/80 antibody (MCA497, clone CI:A3-1) is the original and the best: High specificity – shown to be highly targeted for all mouse macrophages. Added versatility for microglial cells – unlike many other macrophage markers F4/80 is present on microglia which makes it perfect for detecting and isolating these cells in neurological research.
Where are F4 / 80 antigens found in mouse cells?
F4/80 antigen is a mature mouse cell surface glycoprotein expressed at high levels on various macrophages including: Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and Langerhans cells in the skin.
When to use antigen unmasking for F4 / 80 immunostaining?
For use with F4/80 Immunostaining of Paraffin-Embedded Sections. Antigen unmasking procedures are recommended to achieve optimum IHC staining for F4/80, as the F4/80 antigen can be masked by prolonged formalin-fixation and the paraffin-embedding process.