Is Sal 1 a restriction enzyme?
SalI is a restriction endonuclease used for molecular biology methods to cleave DNA at the recognition sequence 5′-G/TCGAC-3′, generating fragments with 5′-cohesive ends.
Is pvu 1 a restriction site?
PvuI is a restriction endonuclease that is used for molecular biological applications to cleave DNA at the recognition sequence 5′-CGAT/CG-3′ to generate fragments with 3′-cohesive termini.
What is cla1?
ClaI is a restriction enzyme that cleaves the DNA recognition sequence 5′-AT/CGAT-3′, generating DNA fragments with 5′-cohesive ends.
What are the differences between type I and type II restriction enzymes?
Type I restriction enzyme possesses a cleaving site which is away from the recognition site. Type II restriction enzymes cleave within the recognition site itself or at a closer distance to it. This is the key difference between Type I and Type II restriction enzyme.
What is the difference between Type II and Type IIS restriction enzymes?
In Type IIP restriction enzymes, the amino acids that catalyze cleavage and those that recognize the DNA are integrated into a single protein domain that cannot be effectively sub-divided. In Type IIS enzymes, in contrast, they are partitioned into separate domains linked by a short polypeptide connector.
Which is E coli strain carries the SFII gene?
An E. coli strain that carries the SfiI gene from Streptomyces fimbriatus (ATCC 15051). One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 50°C in a total reaction volume of 50 µl.
What kind of DNA is used to assay SFII?
Note: Assayed using pUC19 DNA with insert containing two SfiI sites from Adenovirus-2 DNA. Incubation at 37°C results in 10% activity. SfiI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.
Which is the best restriction enzyme from Takara?
DNA Restriction Enzymes from Takara such as SfiI are high-quality: perform restriction enzyme digestion with reliable restriction endonucleases. Cat. # 1244B contains 5 of Cat. # 1244A.
Do you need two copies of the SFII recognition sequence?
For cleavage with SfiI, at least two copies of its recognition sequence are required. The two sites can be on either the same or different DNA molecules. (L. M. Wertzell et al., J. Mol. Biol., 248, 581-595, 1995.) Therefore, also an oligonucleotide harboring a SfiI recognition site can be supplemented.