How is RhIG calculated?
Number of RhIG vials of 300 µg RhIG = fetal cells/total cells counted × maternal blood volume (mL) / 30 mL then rounded to nearest whole number and added 1.
How is Kleihauer-Betke calculated?
V. Interpretation
- Calculate Maternal Blood Volume (ml) = (Pre-pregnant weight in kg) x 70 ml/kg x. (1.0 + (0.5 x weeks gestation/36)) –
- Calculate Fetal Whole Blood (ml) = (Fetal Cell Count/Maternal Cell Count) x. Maternal Blood Volume.
- Rh Immune Globulin (RhoGAM) Dose. Give 300 ug per 30 ml fetal whole blood or 15 ml pRBC.
How many doses of RhIG are administered for a fetal bleed of 25ml?
The standard dose of RhIG is 300 mcg and is increased (300 mcg for every 25 mL of fetal blood in maternal circulation) based on the amount of fetomaternal hemorrhage, which can be quantified using the Kleihauer-Betke technique.
How does RhIG work?
Rh immune globulin (RhIG) is a medication that contains antibodies to Rh antigen D (RhD), which may be present on the surface of red blood cells (RBCs) in some individuals. It prevents a person’s immune system from recognizing RhD, thus suppressing a potentially fatal immune reaction.
What does Rh immune globulin do?
Rh immune globulin (WinRho) is a blood product that is given when you’re at risk of forming antibodies. This can happen if you’re Rh negative and are pregnant, or could someday become pregnant. Antibodies are proteins your body makes to protect itself when it reacts with something that it doesn’t recognize.
How much RhoGAM do you give?
After 12-weeks gestational age, a dose of 300 mcg is recommended. This standard dose of Rho(D) immune globulin (300 mcg) covers FMH up to 15 mL of fetal red cells (30 mL of whole fetal blood).
How is Kleihauer test done?
The Kleihauer Betke test is utilized to determine if there is fetal blood in maternal circulation, with a threshold of 5 mL. The rosette test is performed by incubating the Rh-negative maternal venous whole blood sample with anti-Rho(D) immune globulin.
How do you read Kleihauer?
Kleihauer Betke Assay Interpretation
- One 300 ml vial of RhIG will protect against 30 ml of D-positive fetal blood.
- The conversion factor used to indicate the volume of fetomaternal hemorrhage (as ml’s of whole blood) is: percent fetal red cells observed X 50.
- The calculation for RhIG is as follows:
Why are RhoGAM injections given?
RhoGAM is an immunity support injection administered during pregnancy for people with a negative blood type. The purpose of the RhoGAM shot is to prevent problems that occur due to the baby’s positive blood type. It helps prevent the body from developing Rh antibodies.
What is RhIg candidacy?
RhIG is a concentrated form of an antibody against the main Rh antigen, “D.” It is given to D-negative mothers during pregnancy and in the first 72 hours after delivery of a D-positive baby, in order to prevent them from making anti-D.
What is RhIG injection?
RhoGAM is one brand of Rh immunoglobulin (RhIg). RhIg is an injectable drug given to pregnant people with Rh-negative blood during pregnancy. Every person is born with a blood type—O, A, B, or AB and a Rhesus (Rh) factor, which is positive or negative.