What does FluoroGold stain for?
Fluorogold provides sensitive staining of the axonal and somatodendritic compartments but does not undergo transcytosis.
What is anterograde and retrograde tracing?
Anterograde tracing outlines neurons from their cell bodies to the terminals of their axons; while retrograde tracing outlines neurons in the opposite direction, from the terminals of their axons to their cell bodies. Anterograde and retrograde tracing take advantage of existing transport pathways in neurons.
How do Retrobeads work?
No matter what they call their products, only Retrobeads™ from Lumafluor have been proven effective by hundreds of published papers in systems ranging from invertebrates to primates, and everything in between. Lumafluor Retrobeads™: Highly confined injections–superb for detailed connectivity studies.
What do retrograde tracers do?
Retrograde tracing is a research method used in neuroscience to trace neural connections from their point of termination (the synapse) to their source (the cell body). These techniques allow the “mapping” of connections between neurons in a particular structure (e.g. the eye) and the target neurons in the brain.
What is a retrograde transmitter?
In neuroscience, retrograde signaling (retrograde neurotransmission) is the process by which a retrograde messenger, such as anandamide or nitric oxide, is released by a postsynaptic dendrite or cell body, and travels backwards across a chemical synapse to bind to the axon terminal of a presynaptic neuron.
What is retrograde anatomical labels?
Retrograde tracing (retrograde labeling) is a neuroanatomical method used to determine the location of the cells of origin of a nervous system pathway. A tracer substance that will be taken up by synaptic terminals (and sometimes by axons) is injected into a region of interest, such as a central nervous system nucleus.
What should the shelf life of fluorochrome be?
Dry Fluoro-Gold should be kept in a light tight closed container at 4 degrees Celsius. Stored properly, Fluoro-Gold should have a shelf life exceeding one year. The dye in solution should also be kept in a light tight closed container at 4 degrees Celsius and should remain stable for at least six months.
When was FluoroGold used in computer image processing?
These fluorescence methods were developed in the 1980s and 1990s in parallel with technological advances leading to confocal laser scanning microscopy and related computer image processing.
How is FluoroGold used to treat nerve damage?
Fluorogold can be applied by either pressure or iontophoretic injection into the region of interest, where it is taken up by damaged neurons and at nerve terminals by fluid-phase endocytosis. The advantage of this tracer lies in the availability of specific antibodies to fluorogold.
What should be the concentration of Fluoro Gold?
Fluoro-Gold has been successfully used at concentrations ranging from 1-10%. Initially, a 4% concentration is advised. If undesirable necrosis occurs at the injection site, or labeling is too intense, reduce the concentration to a 2% solution.