What component of a tissue section of the brain does Luxol fast blue stain?
myelin sheath
Luxol fast blue is a copper phthalocyanine dye that is soluble in alcohol and is attracted to bases found in the lipoproteins of the myelin sheath. Under the stain, myelin fibers appear blue, neuropil appears pink, and nerve cells appear purple.
What is the fixative used when using Luxol fast blue PAS hematoxylin stain for myelin?
The myelin, including phospholipids, will be stained blue to green, and the neurons will be stained violet. This stain is commonly used for identifying the basic neuronal structure in brain or spinal cord tissue. Fixation: 10% formalin. Section: paraffin sections at 5-10 um.
What does Luxol fast blue stain?
Luxol fadt blue stain (LFB) is used to visualize myelin in nerve tissue. Thanks to this staining, it is possible to visualize the myelin and the fatty substance of the brain called white matter which includes the axons of the nerve cells.
What is Luxol fast blue stain used for?
Luxol Fast Blue Stain Kit (Myelin Stain) ab150675 is designed for staining myelin/myelinated axons and Nissl bodies on formalin fixed, paraffin-embedded tissue as well as frozen tissue. This product is used for identifying the basic neuronal structure in brain or spinal cord sections.
Does nissl stain glia?
First, the Nissl technique stains the entire population of neurons and glial cell types in the same section. These features make Nissl staining the most suitable technique for labeling neurons and glial cell types in stereological counts of entire nerve cell populations in the cortex.
What does a nissl stain show?
Nissl stain (e.g., cresyl violet, thionin, azure) stains nuclei acids (DNA and RNA). This stain is useful for viewing cell sizes and numbers.
How does myelination increase conduction speed?
Myelin can greatly increase the speed of electrical impulses in neurons because it insulates the axon and assembles voltage-gated sodium channel clusters at discrete nodes along its length.