Is BamHI compatible with BglII?
BamHI and BglII generate different-but-compatible ends. Ligation is possible between both identical ends and between the different-but-compatible ends, but identical-end products will be converted back into substrate by BamHI and BglII digestion. This ligation is performed in Figure 1B.
What is BamHI compatible with?
Cleavage with two restriction endonucleases that produce compatible overhangs….Compatible Cohesive Ends and Generation of New Restriction Sites.
| Enzyme | Ligated To | Recleaved By |
|---|---|---|
| AvrII (C/CTAGG) | NheI, SpeI, XbaI | BfaI |
| StyI (C/CTAGG) | AvrII, BfaI, BsaJI, StyI | |
| BamHI * (G/GATCC) | BclI, DpnII | AlwI, DpnII |
What restriction enzymes are compatible ends?
Cleavage with two restriction endonucleases that produce compatible overhangs….Compatible Cohesive Ends and Generation of New Restriction Sites.
| Enzyme | Ligated To | Recleaved By |
|---|---|---|
| AciI (C/CGC) | BsaHI (GR/CGCC), HinP1I, NarI | AciI |
| HpaII | HpaII | |
| AclI (AA/CGTT) | AccI (GT/CGAC), AciI, ClaI, BstBI, HinP1I, HpaII, NarI, TaqI-v2 | — |
| AgeI * (A/CCGGT) | AvaI (C/CCGGG), XmaI | HpaII, NciI, ScrFI |
What are compatible restriction enzymes?
As unlikely as it may seem, restriction enzymes from different organisms can produce interlocking pieces of DNA – so called compatible cohesive ends (CCE). These are pieces of DNA, which fit together and can be ligated, creating a hybrid molecule.
Does BamHI cut DNA?
In addition to the canonical G decreases from G-A-T-C-C site, BamHI cuts DNA at several sites that we have named noncanonical BamHI.
What can BamHI restriction enzymes be used for?
DNA Restriction Enzymes from Takara such as BamHI are high-quality: perform restriction enzyme digestion with reliable restriction endonucleases. Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind.
What is the function of the BglII enzyme?
The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition. Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix.
How does DNA methylation affect the activity of BamHI?
Effect of DNA methylation: enzyme activity is not affected by CG methylase, dam methylase, or dcm methylase. Reaction temperature: 30°C. DNA Restriction Enzymes from Takara such as BamHI are high-quality: perform restriction enzyme digestion with reliable restriction endonucleases.
Is there a double digestion buffer for BamHI?
I realize that BamHI has a unique buffer. However, based on online search for BamHI and BglII double digestion, the 2X Tango Buffer is recommended. I tried several times but still can’t get a good result.