How to culture HL-60 cells?
HL60 cells should be cultured in RPMI 1640 + 2mM Glutamine + 10-20% Foetal Bovine Serum (FBS). At ECACC it has been found to be difficult to recover cells of acceptable viability after freezing in 10% glycerol/90% FBS.
What is useful about the HL-60 cell line?
HL-60 is one of the large number of cell lines that could be selected as being highly useful in cancer research. HL-60 cells can be used for assessing chemotherapy drugs and, when differentiated, can be used as a model for vaccine targets.
How do you tell the difference between HL and 60 cells?
HL-60 cells were differentiated with 1.3% v/v DMSO for 6 days with antibiotics or 5 μM of ATRA for 4 days without media change. For nuclei staining HL-60 neutrophil-like cells were treated with 0.5 μg/ml Hoechst 33,342 (Life Technologies) for 5 min at 37 °C and washed in PBS once.
What does HL-60 stand for?
human promyelocytic leukemia cell line
HL60 (human promyelocytic leukemia cell line) HL60 is a human caucasian promyelocytic leukemia cell line (Figure 7.3(a)), derived from a 36-year-old woman with acute PML at the National Cancer Institute.
What type of cells are HL-60 cells?
The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals.
How do you subculture a human leukemia cell line HL-60?
Subculture Routine Take a sample of the cell suspension, e.g. 100ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 mintues. Remove medium and resuspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium containing 20% serum.
What is APL disease?
Acute promyelocytic leukemia (APL) is a blood cancer characterized by a marked increase in a type of white blood cells known as promyelocytes, a type of immature white blood cell. It develops in about 600 to 800 individuals each year in the United States, most often in adults around the age of 40.
What are HL 60 cells and why are they interesting in the study of apoptosis?
HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells and is especially useful in dielectrophoresis studies, which require an aqueous environment with suspended and round cells.
Are HL-60 adherent?
HL-60 promyelocytic cells can be induced to differentiate into more mature myeloid cells or macrophages. TPA induc- tion results in a selective adherence of GI and Gz cells and a block at the GI and S border and G2-M stage.
What are HL-60 cells and why are they interesting in the study of apoptosis?
Can you suddenly get leukemia?
Acute leukemia comes on suddenly, and the cancerous cells multiply rapidly. Chronic conditions result from slowly developing cancer cells, and it may take years before a person experiences any symptoms.