What is the principle of UV visible spectroscopy?
PRINCIPLE UV-visible spectroscopy measure the response of a sample to ultra violet and visible range of electromagnetic radiation. Molecules have either n,TC or Electrons. These electrons absorb UV radiation & undergoes transitions from ground state to excited state.
How does the absorption spectrum of UV radiation work?
THE ABSORPTION SPECTRUM The absorption of uv radiation brings about the promotion of an electron from bonding to antibonding orbital. The wavelength of radiation is slowly changed from minimum to maximum in the given region, and the absorbance at every wavelength is recorded.
Which is a feature of the absorption spectrum?
Then a plot of energy absorbed Vs wavelength is called absorption spectrum. The significant features: Xmax (wavelength at which there is a maximum absorption) emax (The intensity of maximum absorption) The UV spectrum depends on solvents concentr i n f olution
What kind of cells are used for UV spectroscopy?
Virtually all UV spectra are recorded solution-phase Cells can be made of plastic, glass or quart Only quartz is transparent in the full 200-700 nm range; plastic and glass are only suitable for visible spectra Concentration (we will cover shortly) is empirically determined A typical sample cell (commonly called a cuvet)
Which is an instrument used to measure UV absorption?
INTRODUCTION Spectroscopy is the branch of Science that deals with the study of interaction of electromagnetic radiation with matter. Spectrometer is an instrument design to measure the spectrum of a compound. Instrument used to measure the absorbance in UV (200- 400nm) or Visible (400-800nm) region is called UV- Visible Spectrophotometer. 2
What kind of material is used in UV spectroscopy?
PRISM Made up of glass,quartz or fused silica. Quartz or fused silica is the choice of material of UV spectrum. When white light is passed through the glass prism, dispersion of polychromatic light in rainbow occurs.Now by the rotation of the prism different wavelengths of the specrum can be made to pass through in exit slit on the sample. 25
How is a UV-VIS spectrometer similar to an IR?
The construction of a traditional UV-VIS spectrometer is very similar to an IR, as similar functions — sample handling, irradiation, detection and output are required Here is a simple schematic that covers most modern UV spectrometers: log (ldl) = A UV-VIS sources 200 monochromator/ beam splitter optics 700