Is CAG a strong promoter?
The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors.
What is the function of CMV promoter?
The CMV promoter is a commonly used promoter for the production of high level recombinant protein in mammalian cells17. However, the expression level of the transgene driven by CMV promoter decreases with extended culture times because of transcriptional silencing, which is associated with DNA methylation18, 19.
What does CAG promoter stand for?
Actually, CAG stands for CMV enhancer, chicken beta-Actin promoter and rabbit beta-Globin splice acceptor site, a combined fusion for very efficient expression in eukaryote cells.
How big is the CAG promoter?
Plasmid: CAG promoter
| Analyze: | Sequence |
|---|---|
| Size: | 584 |
| Notes: | Hybrid construct consisting of the cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter. |
| Stable: | Unspecified |
| Constitutive: | Unspecified |
Does CMV promoter work in bacteria?
These results indicate that bacterial promoter activity, either within, or upstream of, the CMV promoter caused read-through transcription into the C-fragment gene resulting in bacterial expression.
Why is it called cytomegalovirus?
The name is derived from Greek word “cyto” meaning cell and “megalo” meaning big. CMV is also known as HCMV or Human Herpesvirus 5 (HHV-5). CMV belongs to the ”Betaherpesvirinae” subfamily of ”Herpesviridae”, which also includes Roseola virus.
What is pCAG plasmid?
pCAG-Gal4DBD-GBP2 – is a mammalian expression vector designed to express the DBD portion of a GFP-dependent transcription factor. The SV40 ori sequence enables episomal replication of the plasmid in cells expressing the SV40 large-T antigen (e.g., 293T and COS-7 cells).
How does RNA polymerase find the promoter?
RNA polymerases (or associated general transcription factors) are hypothesized to reach promoter sequences by facilitated diffusion (FD). In FD, a protein first binds to nontarget DNA and then reaches the target by a 1D sliding search.
What kind of promoter is the CAG promoter?
CAG promoter is a hybrid construct consisting of the cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter, which is also used to drive transgene expression in different cell lines 26, 27.
How are low copy promoter associated RNAs recognized?
These data demonstrate that low-copy promoter-associated RNAs transcribed through RNAPII promoters are recognized by the antisense strand of the siRNA and function as a recognition motif to direct epigenetic silencing complexes to the corresponding targeted promoters to mediate transcriptional silencing in human cells.
How are promoter-associated RNAs detected in siRNA?
These promoter-associated RNAs were detected by their hybridization to the antisense strand of the complementary promoter-directed siRNA.
How are siRNAs used to silence mutant allele?
Small interfering RNAs (siRNAs) can be designed to specifically and potently target and silence a mutant allele, with little or no effect on the corresponding wild-type allele expression, presenting an opportunity for therapeutic intervention.