What are the three stages of gene expression?
Transcription occurs in the three steps—initiation, elongation, and termination—all shown here.
- Step 1: Initiation. Initiation is the beginning of transcription.
- Step 2: Elongation. Elongation is the addition of nucleotides to the mRNA strand.
- Step 3: Termination.
How does Serial gene analysis work?
Serial analysis of gene expression (SAGE) is a powerful tool, which provides quantitative and comprehensive expression profile of genes in a given cell population. It works by isolating short fragments of genetic information from the expressed genes that are present in the cell being studied.
How do you analyze gene expression?
Most of these techniques, including microarray analysis and reverse transcription polymerase chain reaction (RT-PCR), work by measuring mRNA levels. However, researchers can also analyze gene expression by directly measuring protein levels with a technique known as a Western blot.
What are the four levels of gene expression?
Control of gene expression in eukaryotic cells occurs at epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels.
What are the three important events in the process of transcription explain each?
Transcription takes place in three steps: initiation, elongation, and termination. The steps are illustrated in Figure 2. Initiation is the beginning of transcription. It occurs when the enzyme RNA polymerase binds to a region of a gene called the promoter.
What is the principle of Sage?
Serial analysis of gene expression (SAGE) is based on the principle that an oligonucleotide sequence of 9–10 bp can uniquely identify a gene (18). In this technique, 9-bp oligonucleotides of cDNAs are cloned in a long, concatenated string with “punctuations” between the cDNA oligonucleotides.
What does introns stand for?
An intron (for intragenic region) is any nucleotide sequence within a gene that is removed by RNA splicing during maturation of the final RNA product. In other words, introns are non-coding regions of an RNA transcript, or the DNA encoding it, that are eliminated by splicing before translation.