How to differentiate SH-SY5Y?
Differentiation of SH-SY5Y cells relies on gradual serum deprivation; the addition of retinoic acid, neurotrophic factors and extracellular matrix proteins; and serial splitting to select for differentiated mature adherent neurons.
Are SH-SY5Y cells neurons?
SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.
What are SK-N-SH cells?
SK-N-SH is a neuroblastoma cell line that displays epithelial morphology and grows in adherent culture. Treatment with all-trans-retinoic acid causes these cells to differentiate and adopt a neuronal phenotype, characterized by extensive neurite outgrowth.
Are SH-SY5Y cells adherent?
The cell line SH-SY5Y are semi adherent cell lines, meaning that they have the property to grow as adherent cultures as well as a suspension culture. I would suggest adding Bovine serum albumin to the flask to improve adhesion.
What are human neuroblastoma cells?
Neuroblastoma is a childhood solid tumor composed of primitive cells derived from precursors of the autonomic nervous system.
What is GM12878?
GM12878 is a lymphoblastoid cell line produced from the blood of a female donor with northern and western European ancestry by EBV transformation. It was one of the original HapMap cell lines and has been selected by the International HapMap Project for deep sequencing using the Solexa/Illumina platform.
Where is the subventricular zone?
lateral ventricle
The subventricular zone (SVZ) is one of two regions where neurogenesis persists in the postnatal brain. The SVZ, located along the lateral ventricle, is the largest neurogenic zone in the brain that contains multiple cell populations including astrocyte-like cells and neuroblasts.