What is Qubit for DNA?
Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more specific than UV absorbance. More sensitive than UV absorbance, detecting as little as 10 pg/μl of DNA. Uses as little as 1 μL of sample, even with very dilute samples.
What is the difference between qubit and Nanodrop?
Devices using DNA binding dyes such as the qubit measure DNA concentrations directly, whereas the nanodrop quantifies anything that fluoresces at the approximate wavelength of DNA.
How much does a Qubit fluorometer cost?
Qubit 4 Fluorometer
| db5f4106-b1af-4c5b-a29c-3b103b3ebd19 | |
|---|---|
| Expected Operational Life | Indefinite |
| Unit Cost | $3,135.00 |
| Maintenance Cost | None |
| Tested For Chemical Agents | Not Applicable |
How does Qubit bind to DNA?
The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA.
What does a Qubit do?
The Qubit fluorometer is a lab instrument developed and distributed by Invitrogen (now part of Thermo Fisher) that, among other applications, is used for the quantification of DNA, RNA, and protein.
How does qubit DNA work?
Each dye is specific for one type of molecule (DNA, RNA or protein). The Qubit fluorometer picks this fluorescence signal up and converts it into a DNA concentration measurement by referring to DNA probes of known concentration. It then uses this relationship to calculate the concentration of a sample.
What does a qubit do?
How does the Qubit work?
The Qubit fluorometer uses fluorescent dyes that emit signals only when bound to the specific target molecules (DNA or RNA) even in the presence of free nucleotides, degraded nucleic acids, or protein contaminants.
How does qubit bind to DNA?
How does the Qubit DNA assay kit work?
The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer.
How to calculate the concentration of qubit dsDNA HS?
The Qubit® 2.0 Fluorometer gives values for the Qubit® dsDNA HS Assay in ng/mL. This value corresponds to the concentration after your sample was diluted into the assay tube. To calculate the concentration of your sample, use the following equation: Concentration of your sample = QF value × 200 x
Which is the best qubit assay for single stranded DNA?
The Qubit ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. Common contaminants such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay. The assays are accurate for initial sample concentrations from as little as 250 pg/μL to 10,000 ng/μL.
How are qubits used in nucleic acid quantification?
Sensitive and specific nucleic acid quantification assays. The Qubit assays utilize target-selective dyes that emit fluorescence when bound to DNA, RNA or protein. Unlike UV absorbance, which can overestimate sample concentrations due to contaminants in the sample such as salts, solvents, detergents, proteins, free nucleotides.