What is meant by high performance liquid chromatography?
High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).
What is the principle of high performance liquid chromatography?
The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). Depending on the chemical structure of the analyte, the molecules are retarded while passing the stationary phase.
Why is it called high performance liquid chromatography?
HPLC stands for High Performance Liquid Chromatography. Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography.
How does HPLC instrument work?
Follow the rules and regulations and wear your apron.
- Clean the HPLC.
- Switch it on and wait for it to get started.
- Prepare the instrument for analysis.
- Keep the solvent/solvents in the mobile phase in solvent reservoir or solvent tray.
- Use methanol-water or chloroform-heptane etc as your solvent.
What is high performance liquid chromatography used for?
High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture.
What is high performance liquid chromatography PDF?
Abstract. High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological samples. It is the most versatile, safest, dependable and fastest chromatographic technique for the quality control of drug components.
What are the benefits of high performance liquid chromatography?
The advantages of using HPLC for analysis are that it requires a small sample size, testing can be modified depending on level of quantification needed, and it produces reliable results.
What is the difference between high-pressure and high performance liquid chromatography?
The Differences between LC and HPLC High-performance liquid chromatography (HPLC), also known as high-pressure liquid chromatography, is an advanced type of LC. The difference between traditional LC and HPLC is that the solvent in LC travels by the force of gravity.
How does high performance liquid chromatography differ from regular column chromatography?
High performance liquid chromatography is basically a highly improved form of column chromatography. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres. That makes it much faster.
Which type of high performance liquid chromatography technique is most widely used?
Reversed phase HPLC
Reversed phase HPLC is the most commonly used form of HPLC.