What are the protecting agents used in peptide synthesis?
The most common Ī±-amino-protecting groups for solid-phase peptide synthesis (SPPS) are the 9-fluorenylmethoxycarbonyl (Fmoc) and the tert-butyloxycarbonyl (Boc) groups, used in the Fmoc/tert-butyl (tBu) and Boc/benzyl (Bn) strategies respectively.
Why is DMF used in peptide synthesis?
Dimethylamine is reactive toward the Fmoc protecting group and may remove it. This could result in excess couplings, and accordingly impure products. DMF is cheaper than NMP, so many peptide chemists use it in spite of its tendency to release reactive amines.
Which reactant is not used for peptide synthesis?
Table 18.1. 1
| Vendor | Model | Flow |
|---|---|---|
| Biotage | SAM | N |
| SAP | N | |
| CEM | Liberty | N |
| Liberty1 | N |
Which reagent is used to protect the amino group in peptide synthesis?
Carbamates are useful as protecting groups for amines, and the most commonly employed are -Boc, -Cbz, and -Fmoc. The -Cbz (carboxybenzyl) protecting group was first used by Max Bergmann and Leonidas Zervas in 1932 for the synthesis of peptides, and is sometimes abbreviated ā-Zā in honor of Zervas.
How do you remove Dimethylamine from DMF?
Dimethylamine degradation impurities can be removed by sparging degraded samples with an inert gas such as argon or by sonicating the samples under reduced pressure. As its name indicates, it is a derivative of formamide, the amide of formic acid. DMF is a polar (hydrophilic) aprotic solvent with a high boiling point.
Is DMF soluble in DCM?
DMF is more soluble in DCM , compared to ETOAc. Better try to extract in ETOAc or ether. DMF is soluble in cold or chilled water.
Which coupling reagents can be used in peptide coupling?
The most commonly employed reagents, BOP, PyBOP, and HBTU generate OBt esters, and these have found wide application in routine SPPS and solution synthesis for difficult couplings. Coupling reagents are also available which generate esters that are more reactive than OBt.
Which reagent we use for the protection of amino group?
SOCl2ā / Pyridine.