What are monocyte markers?
Monocytes express typical blood monocyte markers, such as CD14, CD11b, CCR2, and CD16, but at extravascular sites they possess higher levels of CD141, CD11c, HLA-DR, and CCR7, indicating a tissue-imprinted phenotypic change that is reminiscent of DCs (111, 114).
Are monocytes CD11b+?
CD11b+ bone marrow-derived monocytes are the major leukocyte subset responsible for retinal capillary leukostasis in experimental diabetes in mouse and express high levels of CCR5 in the circulation. Am J Pathol. 2012 Aug;181(2):719-27.
Do macrophages express CD16?
Human monocytes express high levels of CD14 and low CD16, while macrophages express low CD14 and high CD16. Additionally, CD68 is a very classic marker for human macrophages.
Do monocytes express CD16?
Human primary monocytes are heterogeneous in terms of phenotype and function, but are sub-divided only based on CD16 and CD14 expression. CD16 expression distinguishes a subset of monocytes with highly pro-inflammatory properties from non-CD16 expressing “classical” monocytes.
What is the normal monocyte count?
The normal absolute monocytes range is between 1 and 10% of the body’s white blood cells. If the body has 8000 white blood cells, then the normal absolute monocytes range is between 80 and 800.
What is CD16 marker?
CD16 is often used as an additional marker to reliably identify different subsets of human immune cells. Several other CD molecules, such as CD11b and CD33, are traditionally used as markers for human myeloid-derived suppressor cells (MDSCs). CD16 allows for distinction between these two types of granulocytes.
What are pro inflammatory monocytes?
Human primary monocytes comprise a heterogeneous population, of which 10-20% can be distinguished by their expression of surface antigen CD161. These CD16+ cells are known as the “inflammatory” subset due to their potent pro-inflammatory activity2.
What is a CD14 monocyte?
Cluster of differentiation 14 (CD14) was described as monocyte/ macrophage differentiation antigen on the surface of myeloid lineage, such as monocytes, macrophages and dendritic cells (DCs).
What is CD16 a marker for?
What are the markers of a monocyte cell?
Monocyte Markers. The exact markers expressed by the monocyte will depend upon the subtype of monocyte and the external stimuli, however monocytes often lack lineage markers for T cells, B cells, NK cells and DC cells, such as: NK1.1, CD90, CD45R and CD11c (Geissmann et al. 2003).
How are monocytes defined in time of flight cytometry?
Approach and results: We use cytometry by time-of-flight mass cytometry to profile human monocytes using a panel of 36 cell surface markers. Using the dimensionality reduction approach visual interactive stochastic neighbor embedding (viSNE), we define monocytes by incorporating all cell surface markers simultaneously.
How are monocyte gating schemes used in clinical studies?
Such a revised gating scheme will be useful for clinical studies of monocyte function in human cardiovascu … Using cytometry by time-of-flight mass cytometry, we have identified a small panel of surface markers that can significantly improve monocyte subset identification and purity in flow cytometry.
What are the subsets of the human monocyte?
Abstract Objective: Human monocyte subsets are defined as classical (CD14++CD16-), intermediate (CD14++CD16+), and nonclassical (CD14+CD16+).