What is HCT 116 cell line?
HCT116 (ATCC® CCL-247™) is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases.
What does ATCC screen cell lines for?
ATCC human cells are invaluable for your public health research. Human cell lines are a critical biological resource. These in vitro models of human biology are used in biomedical research, toxicology studies, bioindustry, drug discovery, vaccine development, protein production, and a host of other applications.
What is mcf7 cell line?
MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers.
What is NCI H460?
The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. Gender Male Karyotype. modal numbr = 57; range = 53 to 65. This is a hypotriploid human cell line.
What is HCT 15 cell line?
Cell Line Description Colorectal adenocarcinoma which is tumorigenic in nude mice.
What is ATCC stands for?
American Type Culture Collection
ATCC (American Type Culture Collection) is a private, nonprofit, global biological resource center and standards organization that provides scientists with the biomaterials and resources they need to conduct critical life science research.
What strains ATCC?
ATCC is the premier source for microbial reference strains. These collections include high-quality, fully authenticated certified reference materials, human microbiome specimens, US National Park Service cultures, and eumycetozoan strains. Our rich portfolio of high-quality materials will move your research forward.
Do MCF-7 cells express HER2?
In most of the MCF7 subclones that contain high EGFR overexpressing cells, the cells with EGFR levels exceeding 80 000 molecules per cell exhibit only moderately elevated levels of HER2 (mostly in the range of 200 000–600 000 molecules per cell).
Why are MCF-7 cells used?
MCF-7 cells are useful for in vitro breast studies because they retained several ideal characteristics particular to mammary epithelium, such as the processing of estrogen, in the form of estradiol, via estrogen receptors (ER) in the cell cytoplasm [28]. It is the first hormone responding breast cancer cell line.
Where can I get a certificate of analysis for HCT 116?
To download a certificate of analysis for HCT 116 ( CCL-247 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of HCT 116 ( CCL-247) is not currently available online.
How long does the warranty for ATCC last?
The product is provided ‘AS IS’ and the viability of ATCC ® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis.
What should be used when handling ATCC cultures?
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen.
Can a HCT 116 cell be positive for keratin?
PubMed: 7214343 The cells are positive for keratin by immunoperoxidase staining. HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression. Check all containers for leakage or breakage.